(Note: Current Rosetta Inpharmatics employees are shown in boldface type.)

Daniel D. Shoemaker, D. A. Lashkari, D. Morris, M. Mittmann, and R. W. Davis.

Abstract

A quantitative and highly parallel method for analyzing deletion mutants has been developed to aid in determining the biological function of thousands of newly identified open reading frames (ORFs) in Saccharomyces cerevisiae. This approach uses a PCR targeting strategy to generate large numbers of deletion strains. Each deletion strain is labeled with a unique 20-base tag sequence that can be detected by hybridization to a high-density oligonucleotide array. The tags serve as unique identifiers (molecular bar codes) that allow analysis of large numbers of deletion strains simultaneously through selective growth conditions. Hybridization experiments show that the arrays are specific, sensitive and quantitative. A pilot study with 11 known yeast genes suggests that the method can be extended to include all of the ORFs in the yeast genome, allowing whole genome analysis with a single selective growth condition and a single hybridization.

Nature Genetics

Comment in: Nat Genet 1996 Dec;14(4):367-70