A microarray platform comparison for neuroscience applications.
J Neurosci Methods. 2004 Jan 15;132(1):57-68.

Mark L. Parrish^a, Nan Wei^b, Sven Duenwald^a, George Y. Tokiwa^a, Yanqun Wang^b, Daniel Holder^b, Hongyue Dai^a, Xiaohua Zhang^b, Christopher Wright^a, Paul Hodor^b, Guy Cavet^a, Robert L. Phillips^b, Benjamin I. Sun^b and Thomas L. Fare^a

^a Rosetta Inpharmatics LLC, a wholly owned subsidiary Merck & Co. Inc., 12040 115th Avenue NE, Kirkland, WA 98034, USA
^b Merck Research Laboratories, Merck & Co. Inc., Sumneytown Pike, West Point, PA 19486-0004, USA

Abstract

To address the need for high sensitivity in gene expression profiling of small neural tissue samples ( approximately 100 ng total RNA), we compared a novel RT-PCR-IVT protocol using fluor-reverse pairs on inkjet oligonucleotide microarrays and an RT-IVT protocol using 33P labeling on nylon cDNA arrays. The comparison protocol was designed to evaluate these systems for sensitivity, specificity, reproducibility, and linearity. We developed parameters, thresholds, and testing conditions that could be used to differentiate various systems that spanned detection chemistry and instrumentation; probe number and selection criteria; and sample processing protocols. We concluded that the inkjet system had better performance in sensitivity, specificity, and reproducibility than the nylon system, and similar performance in linearity. Between these two platforms, the data indicates that the inkjet system would perform better for the transcriptional profiling of 100 ng total RNA samples for neuroscience studies.