Science 287, 873-880 (2000).

(Note: Current Rosetta Inpharmatics employees are shown in boldface type.)

Christopher J. Roberts, Bryce Nelson, Matthew J. Marton, Roland Stoughton, Michael R. Meyer¹, Holly A. Bennett, Yudong He, Hongyue Dai, Wynn L. Walker, Timothy R. Hughes, Mike Tyers, Charles Boone, and Stephen H. Friend

Abstract

Genome-wide transcript profiling was used to monitor signal transduction during yeast pheromone response. Genetic manipulations allowed analysis of changes in gene expression underlying pheromone signaling, cell cycle control, and polarized morphogenesis. A two-dimensional hierarchical clustered matrix, covering 383 of the most highly regulated genes, was constructed from 46 diverse experimental conditions. Diagnostic subsets of coexpressed genes reflected signaling activity, cross talk, and overlap of multiple MAPK pathways. Analysis of the profiles specified by two different MAPKs, Fus3p and Kss1p, revealed functional overlap of the filamentous growth and mating responses. Global transcript analysis reflects biological responses associated with the activation and perturbation of signal transduction pathways.

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